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Presentation
Isolation and Comparative Genomic Analysis of Mycobacteriophage Enkatz
Washington University in St. Louis Undergraduate Research Symposium (2015)
  • Thomas Van Horn, Washington University in St Louis
  • Micah Rickles-Young
  • Shaarada Srivasta, Washington University in St Louis
  • Tina Zudock, Washington University in St Louis
Abstract

Phage Enkatz is a temperate mycobacteriophage isolated from an un-enriched soil sample collected from the South Forty housing area of the Washington University in St. Louis campus. Enkatz displays unequally sized plaques with a clear center that become cloudier with radial distance from the center. Genome analysis indicates that Enkatz is a cluster A1 mycobacteriophage with a genome size of 49,738 bases and 82 identified genes, 33 of which have been assigned functions. This analysis reveals that the majority of the genes in the positive strand code for structural proteins, while the majority of the genes in the negative strand code for various functional enzymes or proteins of unknown function. Comparing Enkatz to Phages Museum and Solon (its closest genetic neighbors) reveals that the positive strand is more conserved than the negative strand. The presence of a Beta-lactamase gene, which has been found to confer antibiotic resistance, suggests that Enkatz may act as a vector to transmit resistance to its bacterial hosts. Research into the Enkatz genome is important because there are 94 distinct A1 Mycobacteriophage, and the minor differences between them can build a picture of phage evolution and how genes are transferred between bacterial hosts.

Keywords
  • Mycobacteriophage,
  • Comparative Genomics
Disciplines
Publication Date
Spring April 17, 2015
Citation Information
Thomas Van Horn, Micah Rickles-Young, Shaarada Srivasta and Tina Zudock. "Isolation and Comparative Genomic Analysis of Mycobacteriophage Enkatz" Washington University in St. Louis Undergraduate Research Symposium (2015)
Available at: http://works.bepress.com/thomas_vanhorn/1/