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Article
Polymorphic microsatellite loci from the western corn rootworm (Insecta: Coleoptera: Chrysomelidae) and cross-amplification with other Diabrotica spp
Molecular Ecology Notes
  • Kyung Seok Kim, Iowa State University
  • Thomas W. Sappington, Iowa State University
Document Type
Article
Publication Date
1-1-2005
DOI
10.1111/j.1471-8286.2004.00853.x
Abstract

Corn rootworms (Diabrotica spp.) make up the major insect pest complex of corn in the US and Europe, and there is a need for molecular markers for genetics studies. We used an enrichment strategy to develop microsatellite markers from the western corn rootworm (Diabrotica virgifera virgifera). Of 54 loci isolated, 25 were polymorphic, and of these, 17 were surveyed for variability in 59 wild individuals. In addition, the potential for cross-amplification of these microsatellites was surveyed for Mexican, northern, and southern corn rootworms. Nine microsatellite loci showed Mendelian inheritance and are likely to be useful in population genetics studies.

Comments

This article is from Molecular Ecology Notes 5 (2005): 115, doi:10.1111/j.1471-8286.2004.00853.x.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-10-03
File Format
application/pdf
Citation Information
Kyung Seok Kim and Thomas W. Sappington. "Polymorphic microsatellite loci from the western corn rootworm (Insecta: Coleoptera: Chrysomelidae) and cross-amplification with other Diabrotica spp" Molecular Ecology Notes Vol. 5 Iss. 1 (2005) p. 115 - 117
Available at: http://works.bepress.com/thomas_sappington/63/