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Article
Isolation and characterization of microsatellite loci from the European corn borer, Ostrinia nubilalis (Hübner) (Insecta: Lepidoptera: Crambidae
Molecular Ecology Resources
  • Kyung Seok Kim, Iowa State University
  • Brad S. Coates, Iowa State University
  • Richard L. Hellmich, Iowa State University
  • Douglas V. Sumerford, Iowa State University
  • Thomas W. Sappington, Iowa State University
Document Type
Article
Disciplines
Publication Date
3-1-2008
DOI
10.1111/j.1471-8286.2007.01974.x
Abstract

Few useful microsatellites are available for population studies of the European corn borer, Ostrinia nubilalis (Hübner). An enrichment strategy was used to develop microsatellite markers for O. nubilalis, and over 500 positive clones were isolated. Seventy-five contained unique microsatellites, 10 of which were polymorphic with discernable polymerase chain reaction products. The 10 loci were surveyed for variability in 72 wild individuals from central Iowa. Five loci showed no deviation from Hardy-Weinberg proportions, and all were successfully cross-amplified in the related Asian corn borer, Ostrinia furnacalis. These loci represent a significant addition to microsatellites appropriate for population studies of O. nubilalis.

Comments

This article is from Molecular Ecology Resources; 8 (2008); 409-411; doi: 10.1111/j.1471-8286.2007.01974.x

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-08-27
File Format
application/pdf
Citation Information
Kyung Seok Kim, Brad S. Coates, Richard L. Hellmich, Douglas V. Sumerford, et al.. "Isolation and characterization of microsatellite loci from the European corn borer, Ostrinia nubilalis (Hübner) (Insecta: Lepidoptera: Crambidae" Molecular Ecology Resources Vol. 8 Iss. 2 (2008) p. 409 - 411
Available at: http://works.bepress.com/thomas_sappington/61/