Article
Regulation of Potassium Transport in Human Lens Epithelial Cells
Experimental Eye Research
Document Type
Article
Publication Date
1-1-2006
Disciplines
Abstract
The major K influx pathways and their response to thiol modification by N-ethylmaleimide (NEM) and protein kinase and phosphatase inhibitors were characterized in human lens epithelial B3 (HLE-B3) cells with Rb as K congener. Ouabain (0.1mM) and bumetanide (5μM) discriminated between the Na/K pump (∼35% of total Rb influx) and Na–K–2Cl cotransport (NKCC) (∼50%). Cl-replacement with nitrate or sulfamate revealed 100μM, activated the Na/K pump and abolished NKCC but did not affect KCC. The data suggest at least partial inverse regulation of KCC and NKCC in HLE-B3 cells by signaling cascades involving serine, threonine and tyrosine phosphorylation/dephosphorylation equilibria.
DOI
10.1016/j.exer.2005.05.002
Citation Information
Peter K. Lauf, Ronald E. Warwar, Thomas L. Brown and Norma C. Adragna. "Regulation of Potassium Transport in Human Lens Epithelial Cells" Experimental Eye Research Vol. 82 Iss. 1 (2006) p. 55 - 64 ISSN: 1096-0007 Available at: http://works.bepress.com/thomas_brown/42/