Glass fiber-reinforced polymer composites have promising applications in infrastructure, marine, and automotive industries due to their low cost, high specific stiffness/strength, durability, and corrosion resistance. Polyurethane (PU) resin system is widely used as matrix material in glass fiber-reinforced composites due to their superior mechanical behavior and higher impact strength. Glass fiber-reinforced PU composites are often manufactured using pultrusion process, due to shorter pot life of PU resin system. in this study, E-glass/PU composites are manufactured using a low-cost vacuum-assisted resin transfer molding process. a novel, one-part PU thermoset resin system with a longer pot life is adopted in this study. Tensile, flexure, and impact tests are conducted on both the thermoset PU neat resin system and E-glass/PU composites. a three-dimensional finite element model is developed in a commercial finite element code to simulate the impact behavior of E-glass/PU composite for three different energy levels. Finite element model is validated by comparing it with experimental results.