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Presentation
Alternatively spliced products of the maize P gene encode proteins with homology to the DNA-binding domain of myb-like transcription factors
Proceedings of the National Academy of Sciences of the United States of America (1991)
  • Erich Grotewold, Cold Spring Harbor Laboratory
  • Prasanna Athma, Cold Spring Harbor Laboratory
  • Thomas Peterson, Cold Spring Harbor Laboratory
Abstract
The Zea mays P gene has been postulated to regulate the biosynthetic pathway of a flavonoid-derived pigment in certain floral tissues [Styles, E. D. & Ceska, O. (1977) Can. J. Genet. Cytol. 19, 289-302]. We have characterized two P transcripts that are alternatively spliced at their 3' ends. One message of 1802 nucleotides encodes a 43.7-kDa protein with an N-terminal region showing approximately 40% homology to the DNA-binding domain of several members of the myb family of protooncogene proteins. A second message of 945 nucleotides encodes a 17.3-kDa protein that contains most of the myb-homologous domain but differs from the first protein at the C terminus. The deduced P-encoded proteins show an even higher homology (70%) in the myb-homologous domain to the maize regulatory gene C1. Additionally, the P and C1 genes are structurally similar in the sizes and positions of the first and second exons and first intron. We show that P is required for accumulation in the pericarp of transcripts of two genes (A1 and C2) encoding enzymes for flavonoid biosynthesis--genes also regulated by C1 in the aleurone.
Keywords
  • pericarp,
  • phlobaphene,
  • flavonoids,
  • regulation,
  • C1 gene
Publication Date
June, 1991
Comments
Copyright 1991 National Academy of Sciences
Citation Information
Erich Grotewold, Prasanna Athma and Thomas Peterson. "Alternatively spliced products of the maize P gene encode proteins with homology to the DNA-binding domain of myb-like transcription factors" Proceedings of the National Academy of Sciences of the United States of America (1991)
Available at: http://works.bepress.com/thomas-peterson/4/