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Article
Transposition-mediated DNA re-replication in maize
eLife
  • Jianbo Zhang, Iowa State University
  • Tao Zuo, Iowa State University
  • Dafang Wang, Iowa State University
  • Thomas Peterson, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
11-18-2014
DOI
10.7554/eLife.03724.001
Abstract

Every DNA segment in a eukaryotic genome normally replicates once and only once per cell cycle to maintain genome stability. We show here that this restriction can be bypassed through alternative transposition, a transposition reaction that utilizes the termini of two separate, nearby transposable elements (TEs). Our results suggest that alternative transposition during S phase can induce re-replication of the TEs and their flanking sequences. The DNA re-replication can spontaneously abort to generate double-strand breaks, which can be repaired to generate Composite Insertions composed of transposon termini flanking segmental duplications of various lengths. These results show how alternative transposition coupled with DNA replication and repair can significantly alter genome structure and may have contributed to rapid genome evolution in maize and possibly other eukaryotes.

Comments

This article is from eLife 3 (2014): e03724, doi: 10.7554/eLife.03724.001. Posted with permission.

Rights
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Copyright Owner
Zhang et al
Language
en
File Format
application/pdf
Citation Information
Jianbo Zhang, Tao Zuo, Dafang Wang and Thomas Peterson. "Transposition-mediated DNA re-replication in maize" eLife Vol. 3 (2014) p. e03724
Available at: http://works.bepress.com/thomas-peterson/12/