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Transposon Ac/Ds -induced chromosomal rearrangements at the rice OsRLG5 locus
Nucleic Acids Research
  • Yuan Hu Xuan, Gyeongsang National University
  • Hai Long Piao, Gyeongsang National University
  • Byoung Il Je, Gyeongsang National University
  • Soon Ju Park, Gyeongsang National University
  • Jin Huang, Gyeongsang National University
  • Jian Bo Zhang, Iowa State University
  • Thomas Peterson, Iowa State University
  • Chang-deok Han, Gyeongsang National University
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Previous studies have shown that pairs of closely-linked Ac/Ds transposable elements can induce various chromosomal rearrangements in plant genomes. To study chromosomal rearrangements in rice, we isolated a line ( OsRLG5-161) that contains two inversely-oriented Ds insertions in OsRLG5 ( Oryza sativa Receptor like kinase Gene 5). Among approximately 300 plants regenerated from OsRLG5-161 heterozygous seeds, 107 contained rearrangements including deletions, duplications and inversions of various sizes. Most rearrangements were induced by previously identified alternative transposition mechanism. Furthermore, we also detected a new class of rearrangements that contain juxtaposed inversions and deletions on the same chromosome. We propose that these novel alleles were generated by a previously unreported type of alternative transposition reactions involving the 5′ and 3′ termini of two inversely-oriented Ds elements located on the same chromatid. Finally, 11% of rearrangements contained inversions resulting from homologous recombination between the two inverted Ds elements in OsRLG5-161 . The high frequency inheritance and great variety of rearrangements obtained suggests that the rice regeneration system results in a burst of transposition activity and a relaxation of the controls which normally limit the transposition competence of individual Ds termini. Together, these results demonstrate a greatly enlarged potential of the Ac/Ds system for plant chromosome engineering.

This article is from Nucleic Acids Research 39 (2011): e149, doi: 10.1093/nar/gkr718. Posted with permission.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Yuan Hu Xuan, Hai Long Piao, Byoung Il Je, Soon Ju Park, et al.. "Transposon Ac/Ds -induced chromosomal rearrangements at the rice OsRLG5 locus" Nucleic Acids Research Vol. 39 Iss. 22 (2011) p. e149
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