Potyviruses cause serious yield losses in maize production worldwide. While the maize dwarf mosaic virus (MDMV) predominates in the USA, sugarcane mosaic virus (SCMV) is a major pathogen in China and Germany. In previous studies, inbred FAP1360A revealed complete resistance against both MDMV and SCMV. Two major SCMV resistance genes, Scmv1 and Scmv2, were located on chromosomes 6 and 3, respectively, in populations derived from crosses with the susceptible inbred line F7. For validation of these results obtained in segregating backcross- or F2:3-populations, near-isogenic lines to F7 have been produced after one initial cross to FAP1360A by repeated backcrossing to F7, phenotypic selection for SCMV resistance, and marker-assisted selection for the Scmv1 and Scmv2 regions from FAP1360A. The near-isogenic line F7R has been studied in detail both at the genomic level and for resistance to different potyviruses. Based on 112 polymorphic simple sequence repeat markers, F7R received genomic segments introgressed from FAP1360A exclusively in the Scmv1 and Scmv2 chromosomal regions. F7R conferred complete resistance to SCMV and MDMV, but also to zea mosaic virus and to systemic infection by wheat streak mosaic virus. FAP1360A, F7, F7R were not systemically infected by high plains virus. Thus, introgression of Scmv1 and Scmv2 from FAP1360A into F7 was sufficient to generate the first potyvirus multiresistant European Flint line reported so far.