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Article
Novel technologies in doubled haploid line development
Plant Biotechnology Journal
  • Jiaojiao Ren, Iowa State University
  • Penghao Wu, Xingjiang Agriculture University
  • Benjamin Trampe, Iowa State University
  • Xiaolong Tian, China Agriculture University
  • Shaojiang Chen, China Agricultural University
  • Thomas Lubberstedt, Iowa State University
Document Type
Article
Publication Date
1-1-2017
DOI
10.1111/pbi.12805
Abstract

haploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter- and intraspecific via chromosome elimination. Here, we focus on haploidization via chromosome elimination, especially the recent advances in centromere-mediated haploidization. Once haploids have been induced, genome doubling is needed to produce DH lines. This study has proposed a new strategy to improve haploid genome doubling by combing haploids and minichromosome technology. With the progress in haploid induction and genome doubling methods, DH technology can facilitate reverse breeding, cytoplasmic male sterile (CMS) line production, gene stacking and a variety of other genetic analysis.

Comments

This article is published as Ren, Jiaojiao, Penghao Wu, Benjamin Trampe, Xiaolong Tian, Shaojiang Chen, and Thomas Lübberstedt. "Novel technologies in doubled haploid line development." Plant Biotechnology Journal (2017). 10.1111/pbi.12805. Posted with permission.

Creative Commons License
Creative Commons Attribution 4.0 International
Copyright Owner
The Authors
Language
en
File Format
application/pdf
Citation Information
Jiaojiao Ren, Penghao Wu, Benjamin Trampe, Xiaolong Tian, et al.. "Novel technologies in doubled haploid line development" Plant Biotechnology Journal Vol. 15 Iss. 11 (2017) p. 1361 - 1370
Available at: http://works.bepress.com/thomas-lubberstedt/42/