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The Soybean Rhg1 Locus for Resistance to the Soybean Cyst Nematode Heterodera glycines Regulates the Expression of a Large Number of Stress- and Defense-Related Genes in Degenerating Feeding Cells
Plant Physiology
  • Pramod Kaitheri Kandoth, University of Missouri
  • Nagabhushana Ithal, University of Missouri
  • Justin Recknor, Iowa State University
  • Tom Maier, Iowa State University
  • Dan Nettleton, Iowa State University
  • Thomas J. Baum, Iowa State University
  • Melissa G. Mitchum, University of Missouri
Document Type
Article
Publication Version
Published Version
Publication Date
4-1-2011
DOI
10.1104/pp.110.167536
Abstract
To gain new insights into the mechanism of soybean (Glycine max) resistance to the soybean cyst nematode (Heterodera glycines), we compared gene expression profiles of developing syncytia in soybean near-isogenic lines differing at Rhg1 (for resistance to Heterodera glycines), a major quantitative trait locus for resistance, by coupling laser capture microdissection with microarray analysis. Gene expression profiling revealed that 1,447 genes were differentially expressed between the two lines. Of these, 241 (16.8%) were stress- and defense-related genes. Several stress-related genes were up-regulated in the resistant line, including those encoding homologs of enzymes that lead to increased levels of reactive oxygen species and proteins associated with the unfolded protein response. These results indicate that syncytia induced in the resistant line are undergoing severe oxidative stress and imbalanced endoplasmic reticulum homeostasis, both of which likely contribute to the resistance reaction. Defense-related genes up-regulated within syncytia of the resistant line included those predominantly involved in apoptotic cell death, the plant hypersensitive response, and salicylic acid-mediated defense signaling; many of these genes were either partially suppressed or not induced to the same level by a virulent soybean cyst nematode population for successful nematode reproduction and development on the resistant line. Our study demonstrates that a network of molecular events take place during Rhg1-mediated resistance, leading to a highly complex defense response against a root pathogen.
Comments

This article is published as Kandoth, Pramod Kaitheri, Nagabhushana Ithal, Justin Recknor, Tom Maier, Dan Nettleton, Thomas J. Baum, and Melissa G. Mitchum. "The Soybean Rhg1 Locus for Resistance to the Soybean Cyst Nematode Heterodera glycines Regulates the Expression of a Large Number of Stress-and Defense-Related Genes in Degenerating Feeding Cells." Plant Physiology 155, no. 4 (2011): 1960-1975, doi: 10.1104/pp.110.167536. Copyright American Society of Plant Biologists. Posted with permission.

Copyright Owner
American Society of Plant Biologists
Language
en
File Format
application/pdf
Citation Information
Pramod Kaitheri Kandoth, Nagabhushana Ithal, Justin Recknor, Tom Maier, et al.. "The Soybean Rhg1 Locus for Resistance to the Soybean Cyst Nematode Heterodera glycines Regulates the Expression of a Large Number of Stress- and Defense-Related Genes in Degenerating Feeding Cells" Plant Physiology Vol. 155 Iss. 4 (2011) p. 1960 - 1975
Available at: http://works.bepress.com/thomas-baum/56/