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Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode Meloidogyne incognita and Expression Analysis During Plant Parasitism
Molecular Plant-Microbe Interactions
  • Marie-Noëlle Rosso, INRA
  • Bruno Favery, INRA
  • Christine Piotte, INRA
  • Laury Arthaud, INRA
  • Jan M. De Boer, Iowa State University
  • Richard S. Hussey, University of Georgia
  • Japp Bakker, Wageningen Agricultural University
  • Thomas J. Baum, Iowa State University
  • Pierre Abad, INRA
Document Type
Article
Publication Version
Published Version
Publication Date
7-1-1999
DOI
10.1094/MPMI.1999.12.7.585
Abstract
A β-1,4-endoglucanase encoding cDNA (EGases, E.C. 3.2.1.4), named Mi-eng-1, was cloned from Meloidogyne incognita second-stage juveniles (J2). The deduced amino acid sequence contains a catalytic domain and a cellulose-binding domain separated by a linker. In M. incognita, the gene is transcribed in the migratory J2, in males, and in the sedentary adult females. In pre-parasitic J2, endoglucanase transcripts are located in the cytoplasm of the subventral esophageal glands. The presence of β-1,4-endoglucanase transcripts in adult females could be related to the expression of the gene in esophageal glands at this stage. However, cellulase activity within the egg matrix of adult females suggests that the endoglucanase may also be synthesized in the rectal glands and involved in the extrusion of the eggs onto the root surface. The maximum identity of the predicted MI-ENG-1 catalytic domain with the recently cloned cyst nematode β-1,4-endoglucanases is 52.5%. In contrast to cyst nematodes, M. incognita pre-parasitic J2 were not found to express a β-1,4-endoglucanase devoid of a cellulose-binding domain.
Comments

This article is published as Rosso, Marie-Noëlle, Bruno Favery, Christine Piotte, Laury Arthaud, Jan M. De Boer, Richard S. Hussey, Jaap Bakker, Thomas J. Baum, and Pierre Abad. "Isolation of a cDNA encoding a β-1, 4-endoglucanase in the root-knot nematode Meloidogyne incognita and expression analysis during plant parasitism." Molecular Plant-Microbe Interactions 12, no. 7 (1999): 585-591, doi: 10.1094/MPMI.1999.12.7.585.

Rights
This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1999.
Language
en
File Format
application/pdf
Citation Information
Marie-Noëlle Rosso, Bruno Favery, Christine Piotte, Laury Arthaud, et al.. "Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode Meloidogyne incognita and Expression Analysis During Plant Parasitism" Molecular Plant-Microbe Interactions Vol. 12 Iss. 7 (1999) p. 585 - 591
Available at: http://works.bepress.com/thomas-baum/51/