In Planta Localization of a β-1,4-Endoglucanase Secreted by Heterodera glycinesMolecular Plant-Microbe Interactions
Publication VersionPublished Version
AbstractPolyclonal sera specific to β-1,4-endoglucanases (cellulases) synthesized in the subventral esophageal gland cells of the soybean cyst nematode, Heterodera glycines, were used to provide the first identification of a nematode esophageal gland protein that is secreted into host plant tissue. Sera generated to proteins encoded by Hg-eng-1 and Hg-eng-2(endoglucanases) did not cross-react with soybean root proteins on Western blots (immunoblots) or in immunofluorescence microscopy of noninoculated (control) soybean root sections. In cross sections of soybean roots at 24 h after inoculation of roots with second-stage juveniles of H. glycines, HG-ENG-1 was localized within the nematode's subventral gland cells and was not detected in root tissue. HG-ENG-2 was localized within the subventral gland cells and was secreted from the juvenile's cortical tissue at 24 h after inoculation of roots with second-stage juveniles of H. glycines. HG-ENG-2 was localized along the juvenile's migratory path through the root cortex.
Copyright OwnerThe American Phytopathological Society
Citation InformationXiaohong Wang, Diane Meyers, Yitang Yan, Thomas Baum, et al.. "In Planta Localization of a β-1,4-Endoglucanase Secreted by Heterodera glycines" Molecular Plant-Microbe Interactions Vol. 12 Iss. 1 (1999) p. 64 - 67
Available at: http://works.bepress.com/thomas-baum/43/