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Article
Differential Display Analysis of the Early Compatible Interaction Between Soybean and the Soybean Cyst Nematode
Molecular Plant-Microbe Interactions
  • Dieter Hermsmeier, Iowa State University
  • Mitra Mazarei, Iowa State University
  • Thomas J. Baum, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
12-1-1998
DOI
10.1094/MPMI.1998.11.12.1258
Abstract
The marked cellular changes during feeding site formation of the soybean cyst nematode (Heterodera glycines) indicate major changes in soybean gene expression. We used differential display of mRNA to detect host gene expression changes during the early compatible interaction between soybean and H. glycines. Fifteen cDNA clones corresponding to mRNAs with different abundances in H. glycines-infected versus uninfected roots were identified. Differential display results indicated that abundances of five mRNAs increased in infected roots, whereas abundances of 10 mRNAs decreased. Transcripts for nine of these 15 cDNAs could be detected on RNA blots, and their hybridization signals confirmed the differential display results for eight of these nine cDNAs. Sequence analyses identified five cDNAs with decreased mRNA levels in infected roots as corresponding to two putative aldolase genes, a transcription-factor TFIIA homologue, the soybean small GTP-binding protein gene sra1, and the soybean auxin down-regulated gene ADR12. RNA blot analyses of other auxin down-regulated genes revealed a decrease in their mRNA abundances in H. glycines-infected roots as well.
Comments

This article is published as Hermsmeier, Dieter, Mitra Mazarei, and Thomas J. Baum. "Differential display analysis of the early compatible interaction between soybean and the soybean cyst nematode." Molecular plant-microbe interactions 11, no. 12 (1998): 1258-1263, doi: 10.1094/MPMI.1998.11.12.1258. Posted with permission.

Copyright Owner
The American Phytopathological Society
Language
en
File Format
application/pdf
Citation Information
Dieter Hermsmeier, Mitra Mazarei and Thomas J. Baum. "Differential Display Analysis of the Early Compatible Interaction Between Soybean and the Soybean Cyst Nematode" Molecular Plant-Microbe Interactions Vol. 11 Iss. 12 (1998) p. 1258 - 1263
Available at: http://works.bepress.com/thomas-baum/42/