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Eighteen New Candidate Effectors of the Phytonematode Heterodera glycines Produced Specifically in the Secretory Esophageal Gland Cells During Parasitism
  • Jason B. Noon, Iowa State University
  • Tarek Hewezi, University of Tennessee, Knoxville
  • Thomas R. Maier, Iowa State University
  • Carl Simmons, DuPont Pioneer
  • Jun-Zhi Wei, DuPont Pioneer
  • Gusui Wu, DuPont Pioneer
  • Victor Llaca, DuPont Pioneer
  • Stéphane Deschamps, DuPont Pioneer
  • Eric L. Davis, North Carolina State University
  • Melissa G. Mitchum, University of Missouri
  • Richard S. Hussey, University of Georgia
  • Thomas J. Baum, Iowa State University
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Heterodera glycines, the soybean cyst nematode, is the number one pathogen of soybean (Glycine max). This nematode infects soybean roots and forms an elaborate feeding site in the vascular cylinder. H. glycines produces an arsenal of effector proteins in the secretory esophageal gland cells. More than 60 H. glycines candidate effectors were identified in previous gland-cell-mining projects. However, it is likely that additional candidate effectors remained unidentified. With the goal of identifying remaining H. glycines candidate effectors, we constructed and sequenced a large gland cell cDNA library resulting in 11,814 expressed sequence tags. After bioinformatic filtering for candidate effectors using a number of criteria, in situ hybridizations were performed in H. glycines whole-mount specimens to identify candidate effectors whose mRNA exclusively accumulated in the esophageal gland cells, which is a hallmark of many nematode effectors. This approach resulted in the identification of 18 new H. glycines esophageal gland-cell-specific candidate effectors. Of these candidate effectors, 11 sequences were pioneers without similarities to known proteins while 7 sequences had similarities to functionally annotated proteins in databases. These putative homologies provided the bases for the development of hypotheses about potential functions in the parasitism process.

This article is published as Noon, J. B., Hewezi, T.,Maier, T. R., Simmons, C.,Wei, J., Wu, G., Llaca, V., Deschamps, S., Davis, E. L., Mitchum, M. G., Hussey, R. S., and Baum, T. J. 2015. Eighteen new candidate effectors of the phytonematode Heterodera glycines produced specifically in the secretory esophageal gland cells during parasitism. Phytopathology 105:1362-1372, doi: 10.1094/PHYTO-02-15-0049-R. Posted with permission.

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The American Phytopathological Society
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Jason B. Noon, Tarek Hewezi, Thomas R. Maier, Carl Simmons, et al.. "Eighteen New Candidate Effectors of the Phytonematode Heterodera glycines Produced Specifically in the Secretory Esophageal Gland Cells During Parasitism" Phytopathology Vol. 105 Iss. 10 (2015) p. 1362 - 1372
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