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Divergent evolution of arrested development in the dauer stage of Caenorhabditis elegans and the infective stage of Heterodera glycines
Genome Biology
  • Axel A Elling, Iowa State University
  • Makedonka Mitreva, Washington University School of Medicine in St. Louis
  • Justin Recknor, Iowa State University
  • Xiaowu Gai, Iowa State University
  • John Martin, Washington University School of Medicine in St. Louis
  • Thomas R Maier, Iowa State University
  • Jeffrey P McDermott, Iowa State University
  • Tarek Hewezi, Iowa State University
  • David McK Bird, North Carolina State University
  • Eric L Davis, North Carolina State University
  • Richard S Hussey, University of Georgia
  • Dan Nettleton, Iowa State University
  • James P McCarter, Washington University School of Medicine in St. Louis
  • Thomas J Baum, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2007
DOI
10.1186/gb-2007-8-10-r211
Abstract
Background: The soybean cyst nematode Heterodera glycines is the most important parasite in soybean production worldwide. A comprehensive analysis of large-scale gene expression changes throughout the development of plant-parasitic nematodes has been lacking to date. Results: We report an extensive genomic analysis of H. glycines, beginning with the generation of 20,100 expressed sequence tags (ESTs). In-depth analysis of these ESTs plus approximately 1,900 previously published sequences predicted 6,860 unique H. glycines genes and allowed a classification by function using InterProScan. Expression profiling of all 6,860 genes throughout the H. glycines life cycle was undertaken using the Affymetrix Soybean Genome Array GeneChip. Our data sets and results represent a comprehensive resource for molecular studies of H. glycines. Demonstrating the power of this resource, we were able to address whether arrested development in the Caenorhabditis elegans dauer larva and the H. glycines infective second-stage juvenile (J2) exhibits shared gene expression profiles. We determined that the gene expression profiles associated with the C. elegans dauer pathway are not uniformly conserved in H. glycines and that the expression profiles of genes for metabolic enzymes of C. elegans dauer larvae and H. glycines infective J2 are dissimilar. Conclusion: Our results indicate that hallmark gene expression patterns and metabolism features are not shared in the developmentally arrested life stages of C. elegans and H. glycines, suggesting that developmental arrest in these two nematode species has undergone more divergent evolution than previously thought and pointing to the need for detailed genomic analyses of individual parasite species.
Comments

This article is published as Elling, Axel A., Makedonka Mitreva, Justin Recknor, Xiaowu Gai, John Martin, Thomas R. Maier, Jeffrey P. McDermott et al. "Divergent evolution of arrested development in the dauer stage of Caenorhabditis elegans and the infective stage of Heterodera glycines." Genome Biology 8, no. 10 (2007): R211, doi; 10.1186/gb-2007-8-10-r211. Posted with permission.

Creative Commons License
Creative Commons Attribution 4.0
Copyright Owner
Elling et al
Language
en
File Format
application/pdf
Citation Information
Axel A Elling, Makedonka Mitreva, Justin Recknor, Xiaowu Gai, et al.. "Divergent evolution of arrested development in the dauer stage of Caenorhabditis elegans and the infective stage of Heterodera glycines" Genome Biology Vol. 8 Iss. 10 (2007) p. R211
Available at: http://works.bepress.com/thomas-baum/40/