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A Profile of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Root-knot Nematode Meloidogyne incognita
Molecular Plant-Microbe Interactions
  • Guozhong Huang, University of Georgia
  • Bingli Gao, University of Georgia
  • Tom Maier, Iowa State University
  • R. Allen, University of Georgia
  • Eric L. Davis, North Carolina State University
  • Thomas J. Baum, Iowa State University
  • Richard S. Hussey, University of Georgia
Document Type
Article
Publication Version
Published Version
Publication Date
5-1-2003
DOI
10.1094/MPMI.2003.16.5.376
Abstract
Identifying parasitism genes encoding proteins secreted from a nematode's esophageal gland cells and injected through its stylet into plant tissue is the key to understanding the molecular basis of nematode parasitism of plants. Meloidogyne incognita parasitism genes were cloned by microaspirating the cytoplasm from the esophageal gland cells of different parasitic stages to provide mRNA to create a gland cell-specific cDNA library by long-distance reverse-transcriptase polymerase chain reaction. Of 2,452 cDNA clones sequenced, deduced protein sequences of 185 cDNAs had a signal peptide for secretion and, thus, could have a role in root-knot nematode parasitism of plants. High-throughput in situ hybridization with cDNA clones encoding signal peptides resulted in probes of 37 unique clones specifically hybridizing to transcripts accumulating within the subventral (13 clones) or dorsal (24 clones) esophageal gland cells of M. incognita. In BLASTP analyses, 73% of the predicted proteins were novel proteins. Those with similarities to known proteins included a pectate lyase, acid phosphatase, and hypothetical proteins from other organisms. Our cell-specific analysis of genes encoding secretory proteins provided, for the first time, a profile of putative parasitism genes expressed in the M. incognita esophageal gland cells throughout the parasitic cycle.
Comments

This article is published as Huang, Guozhong, Bingli Gao, Tom Maier, R. Allen, Eric L. Davis, Thomas J. Baum, and Richard S. Hussey. "A profile of putative parasitism genes expressed in the esophageal gland cells of the root-knot nematode Meloidogyne incognita." Molecular Plant-Microbe Interactions 16, no. 5 (2003): 376-381, doi: 10.1094/MPMI.2003.16.5.376. Posted with permission.

Copyright Owner
The American Phytopathological Society
Language
en
File Format
application/pdf
Citation Information
Guozhong Huang, Bingli Gao, Tom Maier, R. Allen, et al.. "A Profile of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Root-knot Nematode Meloidogyne incognita" Molecular Plant-Microbe Interactions Vol. 16 Iss. 5 (2003) p. 376 - 381
Available at: http://works.bepress.com/thomas-baum/39/