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Article
Developmental Expression of Secretory β-1,4-endoglucanases in the Subventral Esophageal Glands of Heterodera glycines
Molecular Plant-Microbe Interactions
  • Jan M. de Boer, Iowa State University
  • Yitang Yan, North Carolina State University
  • Xiaohong Wang, North Carolina State University
  • Geert Smant, Wageningen Agricultural University
  • Richard S. Hussey, University of Georgia
  • Eric L. Davis, North Carolina State University
  • Thomas J. Baum, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
8-1-1999
DOI
10.1094/MPMI.1999.12.8.663
Abstract
Two β-1,4-endoglucanases (EGases), Hg-eng-1 and Hg-eng-2, were recently cloned from the soybean cyst nematode, Heterodera glycines, and their expression was shown in the subventral esophageal glands of hatched second-stage juveniles (J2). We examined the expression of these EGases in the subventral glands of all post-embryonic life stages of H. glycines by in situ hybridization and immunolocalization. The first detectable accumulation of EGase mRNAs occurred in the subventral glands of unhatched J2. EGase transcripts remained detectable in J2 after hatching and during subsequent root invasion. However, in late parasitic J2 and third-stage juveniles (J3), the percentage of individuals that showed EGase transcripts decreased. In female fourth-stage juveniles and adult females, EGase transcripts were no longer detected in the subventral glands. EGase hybridization signal reappeared in unhatched males coiled within the J3 cuticle, and transcripts were also present in the subventral glands of migratory adult males. Immunofluorescence labeling showed that EGase translation products are most abundantly present in the subventral glands of preparasitic J2, migratory parasitic J2, and adult males. The presence of EGases predominantly in the migratory stages suggests that the enzymes are used by the nematodes to soften the walls of root cells during penetration and intracellular migration.
Comments

This article is published as de Boer, Jan M., Yitang Yan, Xiaohong Wang, Geert Smant, Richard S. Hussey, Eric L. Davis, and Thomas J. Baum. "Developmental expression of secretory β-1, 4-endoglucanases in the subventral esophageal glands of Heterodera glycines." Molecular Plant-Microbe Interactions 12, no. 8 (1999): 663-669, doi: 10.1094/MPMI.1999.12.8.663. Posted with permission.

Copyright Owner
The American Phytopathological Society
Language
en
File Format
application/pdf
Citation Information
Jan M. de Boer, Yitang Yan, Xiaohong Wang, Geert Smant, et al.. "Developmental Expression of Secretory β-1,4-endoglucanases in the Subventral Esophageal Glands of Heterodera glycines" Molecular Plant-Microbe Interactions Vol. 12 Iss. 8 (1999) p. 663 - 669
Available at: http://works.bepress.com/thomas-baum/38/