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The Parasitome of the Phytonematode Heterodera glycines
Molecular Plant-Microbe Interactions
  • Bingli Gao, University of Georgia
  • R. Allen, University of Georgia
  • Tom Maier, Iowa State University
  • Eric L. Davis, North Carolina State University
  • Thomas J. Baum, Iowa State University
  • Richard S. Hussey, University of Georgia
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Parasitism genes expressed in the esophageal gland cells of phytonematodes encode secretions that control the complex process of plant parasitism. In the soybean cyst nematode, Heterodera glycines, the parasitome, i.e., the secreted products of parasitism genes, facilitate nematode migration in soybean roots and mediate the modification of root cells into elaborate feeding cells required to support the growth and development of the nematode. With very few exceptions, the identities of these secretions are unknown, and the mechanisms of cyst nematode parasitism, therefore, remain obscure. The most direct and efficient approach for cloning parasitism genes and rapidly advancing our understanding of the molecular interactions during nematode parasitism of plants is to create gland cell-specific cDNA libraries using cytoplasm microaspirated from the esophageal gland cells of various parasitic stages. By combining expressed sequence tag analysis of a gland cell cDNA library with high throughput in situ expression localization of clones encoding secretory proteins, we obtained the first comprehensive parasitome profile for a parasitic nematode. We identified 51 new H. glycines gland-expressed candidate parasitism genes, of which 38 genes constitute completely novel sequences. Individual parasitome members showed distinct gland cell expression patterns throughout the parasitic cycle. The parasitome complexity discovered paints a more elaborate picture of host cellular events under specific control by the nematode parasite than previously hypothesized.

This article is published as Gao, Bingli, R. Allen, Tom Maier, Eric L. Davis, Thomas J. Baum, and Richard S. Hussey. "The parasitome of the phytonematode Heterodera glycines." Molecular Plant-Microbe Interactions 16, no. 8 (2003): 720-726, doi: 10.1094/MPMI.2003.16.8.720. Posted with permission.

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The American Phytopathological Society
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Bingli Gao, R. Allen, Tom Maier, Eric L. Davis, et al.. "The Parasitome of the Phytonematode Heterodera glycines" Molecular Plant-Microbe Interactions Vol. 16 Iss. 8 (2003) p. 720 - 726
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