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The 8D05 Parasitism Gene of Meloidogyne incognita Is Required for Successful Infection of Host Roots
Phytopathology
  • Binye Xue, North Carolina State University
  • Noureddine Hamamouch, Longwood University
  • Chunying Li, North Carolina State University
  • Guozhong Huang, University of Georgia
  • Richard S. Hussey, University of Georgia
  • Thomas J. Baum, Iowa State University
  • Eric L. Davis, North Carolina State University
Document Type
Article
Publication Version
Published Version
Publication Date
2-1-2013
DOI
10.1094/PHYTO-07-12-0173-R
Abstract
Parasitism genes encode effector proteins that are secreted through the stylet of root-knot nematodes to dramatically modify selected plant cells into giant-cells for feeding. The Mi8D05 parasitism gene previously identified was confirmed to encode a novel protein of 382 amino acids that had only one database homolog identified on contig 2374 within the Meloidogyne apla genome. Mi8D05 expression peaked in M. incognita parasitic second-stage juveniles within host roots and its encoded protein was limited to the subventral esophageal gland cells that produce proteins secreted from the stylet. Constitutive expression of Mi8D05 in transformed Arabidopsis thaliana plants induced accelerated shoot growth and early flowering but had no visible effects on root growth. Independent lines of transgenic Arabidopsis that expressed a double-stranded RNA complementary to Mi8D05 in host-derived RNA interference (RNAi) tests had up to 90% reduction in infection by M. incognita compared with wild-type control plants, suggesting that Mi8D05 plays a critical role in parasitism by the root-knot nematode. Yeast two-hybrid experiments confirmed the specific interaction of the Mi8D05 protein with plant aquaporin tonoplast intrinsic protein 2 (TIP2) and provided evidence that the Mi8D05 effector may help regulate solute and water transport within giant-cells to promote the parasitic interaction.
Comments

This article is published as Xue, B., Hamamouch, N., Li, C., Huang, G., Hussey, R. S., Baum, T. J., and Davis, E. L. 2013. The 8D05 parasitism gene of Meloidogyne incognita is required for successful infection of host roots. Phytopathology 103:175-181, doi; 10.1094/PHYTO-07-12-0173-R. Posted with permission.

Copyright Owner
The American Phytopathological Society
Language
en
File Format
application/pdf
Citation Information
Binye Xue, Noureddine Hamamouch, Chunying Li, Guozhong Huang, et al.. "The 8D05 Parasitism Gene of Meloidogyne incognita Is Required for Successful Infection of Host Roots" Phytopathology Vol. 103 Iss. 2 (2013) p. 175 - 181
Available at: http://works.bepress.com/thomas-baum/28/