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Mining Novel Effector Proteins from the Esophageal Gland Cells of Meloidogyne incognita
Molecular Plant-Microbe Interactions
  • William B. Rutter, Iowa State University
  • Tarek Hewezi, University of Tennessee, Knoxville
  • Sahar Abubucker, Washington University School of Medicine in St. Louis
  • Tom R. Maier, Iowa State University
  • Guozhong Huang, University of Georgia
  • Makedonka Mitreva, Washington University School of Medicine in St. Louis
  • Richard S. Hussey, University of Georgia
  • Thomas J. Baum, Iowa State University
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Meloidogyne incognita is one of the most economically damaging plant pathogens in agriculture and horticulture. Identifying and characterizing the effector proteins which M. incognita secretes into its host plants during infection is an important step toward finding new ways to manage this pest. In this study, we have identified the cDNAs for 18 putative effectors (i.e., proteins that have the potential to facilitate M. incognita parasitism of host plants). These putative effectors are secretory proteins that do not contain transmembrane domains and whose genes are specifically expressed in the secretory gland cells of the nematode, indicating that they are likely secreted from the nematode through its stylet. We have determined that, in the plant cells, these putative effectors are likely to localize to the cytoplasm. Furthermore, the transcripts of many of these novel effectors are specifically upregulated during different stages of the nematode's life cycle, indicating that they function at specific stages during M. incognita parasitism. The predicted proteins showed little to no homology to known proteins from free-living nematode species, suggesting that they evolved recently to support the parasitic lifestyle. On the other hand, several of the effectors are part of gene families within the M. incognita genome as well as that of M. hapla, which points to an important role that these putative effectors are playing in both parasites. With the discovery of these putative effectors, we have increased our knowledge of the effector repertoire utilized by root-knot nematodes to infect, feed on, and reproduce on their host plants. Future studies investigating the roles that these proteins play in planta will help mitigate the effects of this damaging pest.

This article is published as Rutter, William B., Tarek Hewezi, Sahar Abubucker, Tom R. Maier, Guozhong Huang, Makedonka Mitreva, Richard S. Hussey, and Thomas J. Baum. "Mining novel effector proteins from the esophageal gland cells of Meloidogyne incognita." Molecular Plant-Microbe Interactions 27, no. 9 (2014): 965-974, doi: 10.1094/MPMI-03-14-0076-R. Posted with permission.

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The American Phytopathological Society
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Citation Information
William B. Rutter, Tarek Hewezi, Sahar Abubucker, Tom R. Maier, et al.. "Mining Novel Effector Proteins from the Esophageal Gland Cells of Meloidogyne incognita" Molecular Plant-Microbe Interactions Vol. 27 Iss. 9 (2014) p. 965 - 974
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