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The haustorial transcriptomes of Uromyces appendiculatus and Phakopsora pachyrhizi and their candidate effector families
Molecular Plant Pathology
  • Tobias I. Link, Universität Hohenheim
  • Patrick Lang, Universität Konstanz
  • Brian E. Scheffler, United States Department of Agriculture
  • Mary V. Duke, United States Department of Agriculture
  • Michelle A. Graham, United States Department of Agriculture
  • Bret Cooper, United States Department of Agriculture
  • Mark L. Tucker, United States Department of Agriculture
  • Martjin van de Mortel, Iowa State University
  • Ralf T. Voegele, Universität Hohenheim
  • Kurt Mendgen, Universität Konstanz
  • Thomas J. Baum, Iowa State University
  • Steven A. Whitham, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2014
DOI
10.1111/mpp.12099
Abstract
Haustoria of biotrophic rust fungi are responsible for the uptake of nutrients from their hosts and for the production of secreted proteins, known as effectors, which modulate the host immune system. The identification of the transcriptome of haustoria and an understanding of the functions of expressed genes therefore hold essential keys for the elucidation of fungus–plant interactions and the development of novel fungal control strategies. Here, we purified haustoria from infected leaves and used 454 sequencing to examine the haustorial transcriptomes of Phakopsora pachyrhizi and Uromyces appendiculatus, the causal agents of soybean rust and common bean rust, respectively. These pathogens cause extensive yield losses in their respective legume crop hosts. A series of analyses were used to annotate expressed sequences, including transposable elements and viruses, to predict secreted proteins from the assembled sequences and to identify families of candidate effectors. This work provides a foundation for the comparative analysis of haustorial gene expression with further insights into physiology and effector evolution.
Comments

This article is from Molecular Plant Pathology 15 (2014): 379, doi:10.1111/mpp.12099. Posted with permission.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Tobias I. Link, Patrick Lang, Brian E. Scheffler, Mary V. Duke, et al.. "The haustorial transcriptomes of Uromyces appendiculatus and Phakopsora pachyrhizi and their candidate effector families" Molecular Plant Pathology Vol. 15 Iss. 4 (2014) p. 379 - 393
Available at: http://works.bepress.com/thomas-baum/2/