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Article
Identification of potential host plant mimics of CLAVATA3/ESR (CLE)-like peptides from the plant-parasitic nematode Heterodera schachtii
Molecular Plant Pathology
  • Jianying Wang, University of Missouri
  • Amy Replogle, University of Missouri
  • Richard Hussey, University of Georgia
  • Thomas Baum, Iowa State University
  • Xiaohong Wang, United States Department of Agriculture
  • Eric L. Davis, North Carolina State University
  • Melissa G. Mitchum, University of Missouri
Document Type
Article
Publication Version
Published Version
Publication Date
2-1-2011
DOI
10.1111/j.1364-3703.2010.00660.x
Abstract
In this article, we present the cloning of two CLAVATA3/ESR (CLE)-like genes, HsCLE1 and HsCLE2, from the beet cyst nematode Heterodera schachtii, a plant-parasitic cyst nematode with a relatively broad host range that includes the model plant Arabidopsis. CLEs are small secreted peptide ligands that play important roles in plant growth and development. By secreting peptide mimics of plant CLEs, the nematode can developmentally reprogramme root cells for the formation of unique feeding sites within host roots for its own benefit. Both HsCLE1 and HsCLE2 encode small secreted polypeptides with a conserved C-terminal CLE domain sharing highest similarity to Arabidopsis CLEs 1–7. Moreover, HsCLE2 contains a 12-amino-acid CLE motif that is identical to AtCLE5 and AtCLE6. Like all other plant and nematode CLEs identified to date, HsCLEs caused wuschel-like phenotypes when overexpressed in Arabidopsis, and this activity was abolished when the proteins were expressed without the CLE motif. HsCLEs could also function in planta without a signal peptide, highlighting the unique, yet conserved function of nematode CLE variable domains in trafficking CLE peptides for secretion. In a direct comparison of HsCLE2 overexpression phenotypes with those of AtCLE5 and AtCLE6, similar shoot and root phenotypes were observed. Exogenous application of 12-amino-acid synthetic peptides corresponding to the CLE motifs of HsCLEs and AtCLE5/6 suggests that the function of this class of CLEs may be subject to complex endogenous regulation. When seedlings were grown on high concentrations of peptide (10 µm), root growth was suppressed; however, when seedlings were grown on low concentrations of peptide (0.1 µm), root growth was stimulated. Together, these findings indicate that AtCLEs1–7 may be the target peptides mimicked by HsCLEs to promote parasitism.
Comments

This article is published as Wang, Jianying, A. M. Y. Replogle, Richard Hussey, Thomas Baum, Xiaohong Wang, Eric L. Davis, and Melissa G. Mitchum. "Identification of potential host plant mimics of CLAVATA3/ESR (CLE)‐like peptides from the plant‐parasitic nematode Heterodera schachtii." Molecular plant pathology12, no. 2 (2011): 177-186, doi: 10.1111/j.1364-3703.2010.00660.x.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Jianying Wang, Amy Replogle, Richard Hussey, Thomas Baum, et al.. "Identification of potential host plant mimics of CLAVATA3/ESR (CLE)-like peptides from the plant-parasitic nematode Heterodera schachtii" Molecular Plant Pathology Vol. 12 Iss. 2 (2011) p. 177 - 186
Available at: http://works.bepress.com/thomas-baum/19/