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A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max
BMC Research Notes
  • Pramod K. Kandoth, University of Missouri
  • Robert Heinz, University of Missouri
  • Gregory Yeckel, University of Missouri
  • Nathan W. Gross, University of Missouri
  • Parijat S. Juvale, Iowa State University
  • John H. Hill, Iowa State University
  • Steven A. Whitham, Iowa State University
  • Thomas J. Baum, Iowa State University
  • Melissa G. Mitchum, University of Missouri
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Background Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed. Findings Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described. Conclusions The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.

This article is from BMC Research Notes 6 (2013): 1, doi:10.1186/1756-0500-6-255. Posted with permission.

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Kandoth et al.
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Pramod K. Kandoth, Robert Heinz, Gregory Yeckel, Nathan W. Gross, et al.. "A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max" BMC Research Notes Vol. 6 Iss. 255 (2013) p. 1 - 7
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