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A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max
BMC Research Notes
  • Pramod K. Kandoth, University of Missouri
  • Robert Heinz, University of Missouri
  • Gregory Yeckel, University of Missouri
  • Nathan W. Gross, University of Missouri
  • Parijat S. Juvale, Iowa State University
  • John H. Hill, Iowa State University
  • Steven A. Whitham, Iowa State University
  • Thomas J. Baum, Iowa State University
  • Melissa G. Mitchum, University of Missouri
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2013
DOI
10.1186/1756-0500-6-255
Abstract

Background

Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed. Findings

Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described. Conclusions

The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.

Comments

This article is from BMC Research Notes 6 (2013): 1, doi:10.1186/1756-0500-6-255. Posted with permission.

Rights
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright Owner
Kandoth et al.
Language
en
File Format
application/pdf
Citation Information
Pramod K. Kandoth, Robert Heinz, Gregory Yeckel, Nathan W. Gross, et al.. "A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max" BMC Research Notes Vol. 6 Iss. 255 (2013) p. 1 - 7
Available at: http://works.bepress.com/thomas-baum/1/