A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine maxBMC Research Notes
Publication VersionPublished Version
AbstractBackground Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed. Findings Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described. Conclusions The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.
RightsThis article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright OwnerKandoth et al.
Citation InformationPramod K. Kandoth, Robert Heinz, Gregory Yeckel, Nathan W. Gross, et al.. "A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max" BMC Research Notes Vol. 6 Iss. 255 (2013) p. 1 - 7
Available at: http://works.bepress.com/thomas-baum/1/