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Effects of Insulin on Diacylglycerol, Protein Kinase C and Glucose Transport in Rat Skeletal Muscle.
  • B. Yu
  • M. Standaert
  • Thomas Paul Arnold, Nova Southeastern University
  • H. Hermandez
  • j. Watson
  • K. Ways
  • D. R. Cooper
  • R. V. Farese
Conference Title
Date of original Performance / Presentation
Insulin treatment in vivo provoked rapid dose-related increases in diacylglycerol content and/or translocation of protein kinase-C (PKC) from cytosol to membranes in rat soleus and gastrocnemius muscles. These effects were apparent with 1) insulin doses that provoked submaximal and maximal increases in glucose utilization, and 2) glucose-stimulated endogenous insulin secretion. Insulin-stimulated PKC translocation was evident when PKC was assayed by 1) histone or protamine phosphorylation after PKC purification by Mono Q column chromatography, and 2) immunoblotting for PKC beta and PKC epsilon. Dose-related effects of insulin on PKC translocation were also observed in the rat soleus in vitro, and this was associated with increased phosphorylation of 40- and 80-kilodalton proteins, which were also phosphorylated by phorbol ester treatment. A role for diacylglycerol-PKC signalling in insulin-stimulated glucose transport was suggested by studies of [3H]2-deoxyglucose ([3H]2-DOG) uptake in the rat soleus in vitro in that 1) PKC translocation and 2-DOG uptake were correlated; and 2) stimulatory effects of insulin and phorbol esters on 2-DOG uptake were apparently nonadditive.
Citation Information
B. Yu, M. Standaert, Thomas Paul Arnold, H. Hermandez, et al.. "Effects of Insulin on Diacylglycerol, Protein Kinase C and Glucose Transport in Rat Skeletal Muscle." Endocrinology Vol. 130 Iss. 6 p. 3345 - 3355
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