Effects of Insulin and Phorbol Esters on Subcellular distribution of Protein Kinase C isoforms in Rat AdipocytesBiochemistry Journal
ISBN or ISSN1874-091X
Date of original Performance / Presentation1-1-1992
AbstractEffects of insulin and phorbol esters on subcellular distribution of protein kinase C (PKC) isoforms were examined in rat adipocytes. Both agonists provoked rapid decreases in cytosolic, and/or increases in membrane, immunoreactive PKC-alpha, PKC-beta, PKC-gamma, and PKC-epsilon. Effects of phorbol esters on PKC-alpha redistribution to the plasma membrane, however, were much greater than those of insulin. In contrast, insulin, but not phorbol esters, stimulated the translocation of PKC-beta to the plasma membrane, and provoked changes in PKC-zeta redistribution. Neither agonist altered subcellular distribution of PKC-delta, which was detected only in membrane fractions. Our findings indicate that insulin and phorbol esters have overlapping and distinctly different effects on the subcellular redistribution of specific PKC isoforms.
Citation InformationR. V. Farese, M. L. Standaert, Thomas Paul Arnold, A. J. Fransouis, et al.. "Effects of Insulin and Phorbol Esters on Subcellular distribution of Protein Kinase C isoforms in Rat Adipocytes" Biochemistry Journal Vol. 288 p. 319 - 323
Available at: http://works.bepress.com/thomas-arnold/12/