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Article
Effects of Insulin and Phorbol Esters on Subcellular distribution of Protein Kinase C isoforms in Rat Adipocytes
Biochemistry Journal
  • R. V. Farese
  • M. L. Standaert
  • Thomas Paul Arnold, Nova Southeastern University
  • A. J. Fransouis
  • K. Ways
  • H. Hernandez
  • D. R. Cooper
ISBN or ISSN
1874-091X
Date of original Performance / Presentation
1-1-1992
Abstract
Effects of insulin and phorbol esters on subcellular distribution of protein kinase C (PKC) isoforms were examined in rat adipocytes. Both agonists provoked rapid decreases in cytosolic, and/or increases in membrane, immunoreactive PKC-alpha, PKC-beta, PKC-gamma, and PKC-epsilon. Effects of phorbol esters on PKC-alpha redistribution to the plasma membrane, however, were much greater than those of insulin. In contrast, insulin, but not phorbol esters, stimulated the translocation of PKC-beta to the plasma membrane, and provoked changes in PKC-zeta redistribution. Neither agonist altered subcellular distribution of PKC-delta, which was detected only in membrane fractions. Our findings indicate that insulin and phorbol esters have overlapping and distinctly different effects on the subcellular redistribution of specific PKC isoforms.
Citation Information
R. V. Farese, M. L. Standaert, Thomas Paul Arnold, A. J. Fransouis, et al.. "Effects of Insulin and Phorbol Esters on Subcellular distribution of Protein Kinase C isoforms in Rat Adipocytes" Biochemistry Journal Vol. 288 p. 319 - 323
Available at: http://works.bepress.com/thomas-arnold/12/