The toxic and osteolathyrogenic effects of benzoyl hydrazine were evaluated using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX), a rapid and inexpensive in vitro test system. Osteolathyrism, a connective tissue lesion associated with decreased intermolecular bonding in collagen and elastin, is manifested in early embryos as alterations in the long axis of the animal. Morphological examination, including electron microscopy, reveals that gross effects were produced by changes in connective tissue fibers of the notochordal sheath. The effects of benzoyl hydrazine were concentration-, and to a lesser extent developmental stage- (incubation temperature), dependent. The 25C 96- hour LC50 and EC50 values were determined to be 113.68 mg/liter and 58.58 mg/liter, respectively. The 20C 96-hour LC50 and EC50 values were determined to be 671.55 mg/ liter and 85.37 mg/liter, respectively. The 25C and 20C 96-hour teratogenic indices (LC50/EC50) of 1.9 and 7.9, respectively, suggest that benzoyl hydrazine is a developmental hazard.
Available at: http://works.bepress.com/terry_schultz/175/