Artemisia tridentata is an ecologically important shrub in dry habitats of western North America. This species has a large, complex genome due to an evolutionary history with hybridization and whole genome duplications. Aquaporins are an important family of genes that function in water transport. We hypothesize that whole genome duplications in Artemisia tridentata may have allowed for neofunctionalization of Aquaporin genes, allowing for greater drought adaptation. To test this hypothesis, aquaporins in the Artemisia tridentata and Artemisia annua genomes were identified via a BLAST search. Afterward, the upstream promoters were obtained and categorized by categories obtained from a PCA based on gene groupings and subfamilies. We found that the subfamilies of aquaporins in Artemisia tridentata have distinct drivers of expression, and that expression of aquaporins A. tridentata and A. annua are bother primarily driven by light and ABA response.