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Article
Quantification of the Group B Soyasaponins by High-PerformanceLiquid Chromatography
Journal of Agricultural and Food Chemistry
  • Jiang Hu, Iowa State University
  • Sun-Ok Lee, Iowa State University
  • Suzanne Hendrich, Iowa State University
  • Patricia A. Murphy, Iowa State University
Document Type
Article
Publication Date
3-26-2002
DOI
10.1021/jf0114740
Abstract
High-performance liquid chromatographic methods were developed for the isolation and quantitative determination of the group B soyasaponins, including 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran- 4-one (DDMP)-conjugated soyasaponins Rg, f3g, and f3a, and their non-DDMP counterparts, soyasaponins V, I, and II, respectively, with formononetin used as the internal standard. The limits of quantification for soy products were 0.11-4.86 µmol/g. The within-day and between-days assay coefficients of variation were <9.8 and < 14.3%, respectively. The group B soyasaponin concentrations in 46 soybean varieties ranged from 2.50 to 5.85 µmol/g. Soy ingredients (soybean flour, toasted soy hypocotyls, soy protein isolates, textured vegetable protein, soy protein concentrates, and Novasoy) and soy foods (commercial soy milk, tofu, and tempeh) contained the group B soyasaponins from 0.20 to 114.02 µmol/g. There was no apparent correlation between isoflavone and soyasaponin concentrations in the soy products examined.
Comments

Reprinted with permission from Journal of Agricultural and Food Chemistry,50(9):2587-2594. doi: 10.1021/jf0114740. Copyright 2002 American Chemical Society.

Rights
On-time permission is granted only for the use specified in your request. No additional uses are granted (such as derivative works or other editions).
Copyright Owner
American Chemical Society
Language
en
Date Available
2014-11-14
File Format
application/pdf
Citation Information
Jiang Hu, Sun-Ok Lee, Suzanne Hendrich and Patricia A. Murphy. "Quantification of the Group B Soyasaponins by High-PerformanceLiquid Chromatography" Journal of Agricultural and Food Chemistry Vol. 50 Iss. 9 (2002) p. 2587 - 2594
Available at: http://works.bepress.com/suzanne_hendrich/22/