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Article
Human Fecal Metabolism of Soyasaponin I
Journal of Agricultural and Food Chemistry
  • Jiang Hu, Iowa State University
  • Yan L. Zheng, Iowa State University
  • Walter Hyde, Iowa State University
  • Suzanne Hendrich, Iowa State University
  • Patricia A. Murphy, Iowa State University
Document Type
Article
Publication Date
4-6-2004
DOI
10.1021/jf035290s
Abstract

The metabolism of soyasaponin I (3-O-[alpha-L-rhamnopyranosyl-beta-D-galactopyranosyl-beta-D-glucuronopyranosyl]olean-12-ene-3beta,22beta,24-triol) by human fecal microorganisms was investigated. Fresh feces were collected from 15 healthy women and incubated anaerobically with 10 mmol soyasaponin I/g feces at 37 degrees C for 48 h. The disappearance of soyasaponin I in this in vitro fermentation system displayed apparent first-order rate loss kinetics. Two distinct soyasaponin I degradation phenotypes were observed among the subjects: rapid soyasaponin degraders with a rate constant k = 0.24 +/- 0.04 h(-)(1) and slow degraders with a k = 0.07 +/- 0.02 h(-)(1). There were no significant differences in the body mass index, fecal moisture, gut transit time, and soy consumption frequency between the two soyasaponin degradation phenotypes. Two primary gut microbial metabolites of soyasaponin I were identified as soyasaponin III (3-O-[beta-D-galactopyranosyl-beta-D-glucuronopyranosyl]olean-12-ene-3beta,22beta,24-triol) and soyasapogenol B (olean-12-ene-3beta,22beta,24-triol) by NMR and electrospray ionized mass spectroscopy. Soyasaponin III appeared within the first 24 h and disappeared by 48 h. Soyasapogenol B seemed to be the final metabolic product during the 48 h anaerobic incubation. These results indicate that dietary soyasaponins can be metabolized by human gut microorganisms. The sugar moieties of soyasaponins seem to be hydrolyzed sequentially to yield smaller and more hydrophobic metabolites.

Comments

Reprinted with permission from Journal of Agricultural and Food Chemistry, 52(9):2689-2696. doi: 10.1021/jf035290s. Copyright 2004 American Chemical Society.

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On-time permission is granted only for the use specified in your request. No additional uses are granted (such as derivative works or other editions).
Copyright Owner
American Chemical Society
Language
en
Date Available
2014-11-14
File Format
application/pdf
Citation Information
Jiang Hu, Yan L. Zheng, Walter Hyde, Suzanne Hendrich, et al.. "Human Fecal Metabolism of Soyasaponin I" Journal of Agricultural and Food Chemistry Vol. 52 Iss. 9 (2004) p. 2689 - 2696
Available at: http://works.bepress.com/suzanne_hendrich/18/