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protein glycosylation analysis
(2006)
  • Susan Rey
Abstract
protein glycosylation analysis, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs. Glycosylation is critical for a wide range of biological processes, including cell attachment to the extracellular matrix and protein-ligand interactions in the cell. This PTM is characterized by various glycosidic linkages, including N-, O- and C-linked glycosylation, glypiation (GPI anchor attachment) and phosphoglycosylation. Glycoproteins can be detected, purified and analyzed by different strategies, including glycan staining and visualization, glycan cross-linking to agarose or magnetic resin for labeling or purification, or proteomic analysis by mass spectrometry, respectively.
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Publication Date
2006
Citation Information
Susan Rey. "protein glycosylation analysis" (2006)
Available at: http://works.bepress.com/susan_rey/36/