Skip to main content
Associations of Interferon-γ Genotype and Protein Level with Antibody Response Kinetics in Chickens
Avian Diseases
  • H. Zhou, Iowa State University
  • Hyun S. Lillehoj, United States Department of Agriculture
  • Susan J. Lamont, Iowa State University
Document Type
Publication Version
Published Version
Publication Date
Although previous studies have demonstrated an association between interferon-gamma (IFN-gamma) promoter genotype and antibody response kinetics in chickens, the protein levels that may mediate such a gene-trait association have not been determined. The objective of this study, therefore, was to determine the correlation of circulating IFN-gamma levels with both the IFN-gammaIFN-gamma promoter polymorphisms and antibody response in order to evaluate the potential role of IFN-gamma protein in mediating genetic control of antibody response in chickens. Antibody response after Salmonella enteritidis (SE) vaccination at day 10, antibody response to sheep red blood cells (SRBCs) and killed Brucella abortus after immunizations at 19 wk and 22 wk, and serum IFN-gamma protein level were measured in an F2 population derived from inbred lines. A single nucleotide polymorphism in the IFN-gamma promoter region was associated with IFN-gamma protein expression as measured by an enzyme-linked immunosorbent assay after both primary and secondary immunizations. Higher IFN-gamma protein level was correlated with higher antibody level to SE and with increased maximum level and decreased time to reach the maximum secondary antibody response to SRBCs. These results suggest that one of the mechanisms by which promoter polymorphism of IFN-gamma affects antibody production in chickens may involve the circulating level of IFN-gamma protein.

This article is from Avian Diseases 46 (2002): 869.

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
File Format
Citation Information
H. Zhou, Hyun S. Lillehoj and Susan J. Lamont. "Associations of Interferon-γ Genotype and Protein Level with Antibody Response Kinetics in Chickens" Avian Diseases Vol. 46 Iss. 4 (2002) p. 869 - 876
Available at: