Skip to main content
Article
Functional Characterization of a Chicken Major Histocompatibility Complex Class II B Gene Promoter
Immunogenetics
  • Yunfei Chen, Iowa State University
  • Hyun S. Lillehoj, United States Department of Agriculture
  • Chung-Hsin Hsu, Iowa State University
  • Susan L. Carpenter, Iowa State University
  • Susan J. Lamont, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-1997
Abstract

A 0.7 kilobase (kb) DNA fragment from the 5' flanking region of a chicken major histocompatibility complex (MHC) class II B gene was cloned into chloramphenicol acetyltransferase (CAT) reporter vectors and was transfected into a chicken macrophage cell line that expresses a low level of MHC class II antigens. Positive orientation-dependent promoter activity of the chicken DNA was evident in a reporter construct containing an SV40 enhancer. Deletion analysis of this 0.7 kb DNA fragment revealed a short fragment in the 3' end that was crucial for the promoter function and negative regulatory elements (NRE) located further upstream. The conserved MHC class II X and Y boxes did not have a significant effect on promoter activity. Sequence analysis of the 0.7 kb class II B gene upstream region suggests possible involvement of interferon (IFN), E twenty-six specific (ETS)-related proteins, and other factors in regulating this promoter. A chicken T-cell line culture supernatant increased surface expression of MHC class II antigens, as well as class II promoter activity, in this macrophage cell line. This first functional characterization of a chicken MHC class II B gene promoter will aid in understanding the regulatory mechanisms that control the expression of these genes.

Comments

This article is from Immunogenetics 45 (1997): 242.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Yunfei Chen, Hyun S. Lillehoj, Chung-Hsin Hsu, Susan L. Carpenter, et al.. "Functional Characterization of a Chicken Major Histocompatibility Complex Class II B Gene Promoter" Immunogenetics Vol. 45 Iss. 4 (1997) p. 242 - 248
Available at: http://works.bepress.com/susan_lamont/82/