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Functional Characterization of a Chicken Major Histocompatibility Complex Class II B Gene Promoter
  • Yunfei Chen, Iowa State University
  • Hyun S. Lillehoj, United States Department of Agriculture
  • Chung-Hsin Hsu, Iowa State University
  • Susan L. Carpenter, Iowa State University
  • Susan J. Lamont, Iowa State University
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A 0.7 kilobase (kb) DNA fragment from the 5' flanking region of a chicken major histocompatibility complex (MHC) class II B gene was cloned into chloramphenicol acetyltransferase (CAT) reporter vectors and was transfected into a chicken macrophage cell line that expresses a low level of MHC class II antigens. Positive orientation-dependent promoter activity of the chicken DNA was evident in a reporter construct containing an SV40 enhancer. Deletion analysis of this 0.7 kb DNA fragment revealed a short fragment in the 3' end that was crucial for the promoter function and negative regulatory elements (NRE) located further upstream. The conserved MHC class II X and Y boxes did not have a significant effect on promoter activity. Sequence analysis of the 0.7 kb class II B gene upstream region suggests possible involvement of interferon (IFN), E twenty-six specific (ETS)-related proteins, and other factors in regulating this promoter. A chicken T-cell line culture supernatant increased surface expression of MHC class II antigens, as well as class II promoter activity, in this macrophage cell line. This first functional characterization of a chicken MHC class II B gene promoter will aid in understanding the regulatory mechanisms that control the expression of these genes.

This article is from Immunogenetics 45 (1997): 242.

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Yunfei Chen, Hyun S. Lillehoj, Chung-Hsin Hsu, Susan L. Carpenter, et al.. "Functional Characterization of a Chicken Major Histocompatibility Complex Class II B Gene Promoter" Immunogenetics Vol. 45 Iss. 4 (1997) p. 242 - 248
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