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Article
Analysis of Restriction Fragment Length Polymorphisms of the Major Histocompatibility Complex of 15I5-B-Congenic Chicken Lines
Poultry Science
  • Susan J. Lamont, Iowa State University
  • Bradley M. Gerndt, Iowa State University
  • C. M. Warner, Iowa State University
  • Larry Donald Bacon, United States Department of Agriculture
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-1990
DOI
10.3382/ps.0691195
Abstract

Eight 15I5 B-congenic White Leghorn chicken lines, containing haplotypesB 2 , B 5 , B 12, B 13, B 15 , B 19 , and B 21, were subjected to molecular genotyping with chicken B-F (Class I) and B-L (Class II) major histocompatibility complex (MHC) probes. Genomic DNA was digested with restriction enzymes, hybridized with a Class I or Class II probe, and analyzed for restriction fragment length polymorphisms. Digestion withHindIII or EcoRI yielded no B-L polymorphisms. Digestion with PvuII or BglII and hybridization with a B-L or B-F probe produced polymorphisms that were shared between several haplotypes, although the haplotypes with similar patterns were clustered differently between Class I and Class II probes. The genetic variation seen for B-L and B-F probe hybridization ofPvuII digests was much less than that previously demonstrated for B-Gprobing of PvuII digests of the same lines. Description of MHC Class I and II restriction patterns of the well-characterized 15I5 B-congenic lines will aid in identification of genes important in disease resistance.

Comments

This article is from Poultry Science 69 (1990): 1195, doi:10.3382/ps.0691195.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Susan J. Lamont, Bradley M. Gerndt, C. M. Warner and Larry Donald Bacon. "Analysis of Restriction Fragment Length Polymorphisms of the Major Histocompatibility Complex of 15I5-B-Congenic Chicken Lines" Poultry Science Vol. 69 Iss. 7 (1990) p. 1195 - 1203
Available at: http://works.bepress.com/susan_lamont/79/