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Microsatellite Markers Associated with Resistance to Marek’s Disease in Commercial Layer Chickens
Poultry Science
  • Joseph Paul McElroy, Iowa State University
  • Jack C. Dekkers, Iowa State University
  • J. E. Fulton, Hy-Line International
  • Neil P. O'Sullivan, Hy-Line International
  • M. Soller, The Hebrew University of Jerusalem
  • E. Lipkin, The Hebrew University of Jerusalem
  • W. Zhang, Iowa State University
  • Kenneth J. Koehler, Iowa State University
  • Susan J. Lamont, Iowa State University
  • H. H. Cheng, United States Department of Agriculture
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The objective of the current study was to identify QTL conferring resistance to Marek's disease (MD) in commercial layer chickens. To generate the resource population, 2 partially inbred lines that differed in MD-caused mortality were intermated to produce 5 backcross families. Vaccinated chicks were challenged with very virulent plus (vv+) MD virus strain 648A at 6 d and monitored for MD symptoms. A recent field isolate of the MD virus was used because the lines were resistant to commonly used older laboratory strains. Selective genotyping was employed using 81 microsatellites selected based on prior results with selective DNA pooling. Linear regression and Cox proportional hazard models were used to detect associations between marker genotypes and survival. Significance thresholds were validated by simulation. Seven and 6 markers were significant based on proportion of false positive and false discovery rate thresholds less than 0.2, respectively. Seventeen markers were associated with MD survival considering a comparison-wise error rate of 0.10, which is about twice the number expected by chance, indicating that at least some of the associations represent true effects. Thus, the present study shows that loci affecting MD resistance can be mapped in commercial layer lines. More comprehensive studies are under way to confirm and extend these results.

This article is from Poultry Science 84 (2005): 1678, doi:10.1093/ps/84.11.1678.

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
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Joseph Paul McElroy, Jack C. Dekkers, J. E. Fulton, Neil P. O'Sullivan, et al.. "Microsatellite Markers Associated with Resistance to Marek’s Disease in Commercial Layer Chickens" Poultry Science Vol. 84 Iss. 11 (2005) p. 1678 - 1688
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