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Mapping and Genotypic Analysis of the NK-Lysin Gene in Chicken
Genetics Selection Evolution
  • Mi Ok Lee, Texas A & M University - College Station
  • Ence Yang, Texas A & M University - College Station
  • Mireille Morisson, Université de Toulouse
  • Alain Vignal, Université de Toulouse
  • Yong-Zhen Huang, Northwest A&F University
  • Hans H. Cheng, United States Department of Agriculture
  • William M. Muir, Purdue University
  • Susan J. Lamont, Iowa State University
  • Hyun Soon Lillehoj, United States Department of Agriculture
  • Sung Hyen Lee, United States Department of Agriculture
  • James E. Womack, Texas A & M University - College Station
Document Type
Article
Publication Version
Published Version
Publication Date
7-1-2014
DOI
10.1186/1297-9686-46-43
Abstract
Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken NK-lysin gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the NK-lysin gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds. A 6000 rad radiation hybrid panel (ChickRH6) was used to map the NK-lysin gene to the distal end of chromosome 22. Two additional genes, the adipocyte enhancer-binding protein 1-like gene (AEBP1) and the DNA polymerase delta subunit 2-like (POLD2) gene, are located in the same NW_003779909 contig as NK-lysin, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines. The chicken NK-lysin gene mapped to the distal end of chromosome 22. Two additional genes,AEBP1 and POLD2, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.
Comments

This article is from Genetics Selection Evolution 46 (2014): 43, doi:10.1186/1297-9686-46-43.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Mi Ok Lee, Ence Yang, Mireille Morisson, Alain Vignal, et al.. "Mapping and Genotypic Analysis of the NK-Lysin Gene in Chicken" Genetics Selection Evolution Vol. 46 (2014) p. 1 - 7
Available at: http://works.bepress.com/susan_lamont/56/