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A Systematic Analysis of miRNA Transcriptome in Marek’s Disease Virus-Induced Lymphoma Reveals Novel and Differentially Expressed miRNAs
PLoS One
  • Ling Lian, China Agricultural University
  • Lujiang Qu, China Agricultural University
  • Yanmei Chen, China Agricultural University
  • Susan J. Lamont, Iowa State University
  • Ning Yang, China Agricultural University
Document Type
Article
Publication Version
Published Version
Publication Date
11-1-2012
DOI
10.1371/journal.pone.0051003
Abstract
Marek’s disease is a lymphoproliferative neoplastic disease of the chicken, which poses a serious threat to poultry health. Marek’s disease virus (MDV)-induced T-cell lymphoma is also an excellent biomedical model for neoplasia research. Recently, miRNAs have been demonstrated to play crucial roles in mediating neoplastic transformation. To investigate host miRNA expression profiles in the tumor transformation phase of MDV infection, we performed deep sequencing in two MDVinfected samples (tumorous spleen and MD lymphoma from liver), and two non-infected controls (non-infected spleen and lymphocytes). In total, 187 and 16 known miRNAs were identified in chicken and MDV, respectively, and 17 novel chicken miRNAs were further confirmed by qPCR. We identified 28 down-regulated miRNAs and 11 up-regulated miRNAs in MDVinfected samples by bioinformatic analysis. Of nine further tested by qPCR, seven were verified. The gga-miR-181a, gga-miR- 26a, gga-miR-221, gga-miR-222, gga-miR-199*, and gga-miR-140* were down-regulated, and gga-miR-146c was upregulated in MDV-infected tumorous spleens and MD lymphomas. In addition, 189 putative target genes for seven differentially expressed miRNAs were predicted. The luciferase reporter gene assay showed interactions of gga-miR-181a with MYBL1, gga-miR-181a with IGF2BP3, and gga-miR-26a with EIF3A. Differential expression of miRNAs and the predicted targets strongly suggest that they contribute to MDV-induced lymphomagenesis.
Comments

This article is from PLoS One 7 (2012): eS1003, doi:10.1371/journal.pone.0051003. Posted with permisison.

Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Owner
Ling Lian, et al
Language
en
File Format
application/pdf
Citation Information
Ling Lian, Lujiang Qu, Yanmei Chen, Susan J. Lamont, et al.. "A Systematic Analysis of miRNA Transcriptome in Marek’s Disease Virus-Induced Lymphoma Reveals Novel and Differentially Expressed miRNAs" PLoS One Vol. 7 Iss. 11 (2012) p. 1 - 13
Available at: http://works.bepress.com/susan_lamont/55/