DNA Methylome in Spleen of Avian Pathogenic Escherichia coli-Challenged Broilers and Integration with mRNA ExpressionScientific Reports
Publication VersionPublished Version
AbstractAvian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD) and severe pathology (SV). DNA methylation was enriched in the gene bodies and repeats. Promoter and CGIs are hypomethylated. Integration analysis revealed 22, 87, and 9 genes exhibiting inversely changed DNA methylation and gene expression in NC vs. MD, NC vs. SV, and MD vs. SV, respectively.IL8, IL2RB, and IL1RAPL1 were included. Gene network analysis suggested that besides inflammatory response, other networks and pathways such as organismal injury and abnormalities, cell signaling and molecular transport, are probably related to host response to APEC infection. Moreover, methylation changes in cell cycle processes might contribute to the lesion phenotype differences between MD and SV.
RightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright OwnerHaiping Xu, et al
Citation InformationHaiping Xu, Xuenong Zhu, Yongsheng Hu, Zhenhui Li, et al.. "DNA Methylome in Spleen of Avian Pathogenic Escherichia coli-Challenged Broilers and Integration with mRNA Expression" Scientific Reports Vol. 4 (2014) p. 1 - 10
Available at: http://works.bepress.com/susan_lamont/53/