Gene Knock-Down in Chicken Immune CellsAnimal Industry Report
Extension NumberASL R2328
Summary and ImplicationsChicken macrophages, when treated with inducible nitric oxide synthase (iNOS) short interfering RNA (siRNA) and then stimulated with recombinant chicken IFN-γ, produced significantly less nitric oxide (NO) and had lower iNOS mRNA levels compared to IFN-γ stimulated HD-11 cells not treated with siRNA. As gene knock-outs are not readily available for most agricultural species, such as the chicken, siRNA technology to reduce gene expression could prove to be a powerful tool in advancing basic knowledge of avian immune function and immune response to infection. Our novel demonstration of siRNA-mediated knock-down of iNOS mRNA expression and NO production in HD-11 macrophages establishes the validity and feasibility of using RNAi technology in the avian immune system, thus providing a foundation for future investigations in avian immune function and the chicken immune response to bacterial pathogens of economic importance such as Salmonella enteritidis.
Copyright HolderIowa State University
Citation InformationJennifer H. Cheeseman and Susan J. Lamont. "Gene Knock-Down in Chicken Immune Cells" (2008)
Available at: http://works.bepress.com/susan_lamont/37/