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Unpublished Paper
The Regulatory Mechanism of Response to CpG-ODN, a Pathogen-derived Molecule, in Chicken Macrophages
Animal Industry Report
  • Ceren Ciraci, Iowa State University
  • Susan J. Lamont, Iowa State University
Extension Number
ASL R2617
Publication Date
2011
Disciplines
Topic
Poultry
Summary and Implications

The regulation and mechanism of action of the avianspecific Toll-like receptors (TLR) genes have been characterized in chicken macrophages in the present study. Chicken TLR15 is a novel pattern recognition receptor whose cognate ligand is unknown and is structurally different from other TLRs. Screening various stimulants in macrophage cell cultures, we concluded that TLR15, in collaboration with TLR2 and TLR21, is involved in CpG oligonucleotide (ODN) response in chicken macrophages. We further verified CpG responsiveness by using inhibitor ODN in macrophage cells. Additionally, we determined the pathway use of MyD88 adaptor molecule after stimulation with CpG-ODNs, resulting in IL1B expression. The current study expands the possibilities for vaccine development studies by discovering disease response pathways.

Copyright Holder
Iowa State University
Language
en
Citation Information
Ceren Ciraci and Susan J. Lamont. "The Regulatory Mechanism of Response to CpG-ODN, a Pathogen-derived Molecule, in Chicken Macrophages" (2011)
Available at: http://works.bepress.com/susan_lamont/30/