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Article
Rhamnolipid from Pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes.
Cell motility and the cytoskeleton
  • Annette T. Hastie
  • Susan T. Hingley, Philadelphia College of Osteopathic Medicine
  • M. L. Higgins
  • Friedrich Kueppers
  • T. Shryock
Document Type
Article
Publication Date
1-1-1986
Abstract
Isolated ciliary axonemes from pig trachea were exposed to increasing concentrations of purified Pseudomonas aeruginosa rhamnolipid. This is a defined ciliary system allowing observation of direct impairment of functional axonemes. Axonemal motility and ATPase activity were decreased in proportion to rhamnolipid concentrations. ATPase-associated proteins observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dynein arms seen in ultra-structural cross sections progressively disappeared from axonemes with exposure to rhamnolipid. These four independent measures establish that the rhamnolipid removes the ATPase-containing outer dynein arms from the ciliary axoneme, thereby rendering the axoneme immotile.
Comments

This article was published in Cell motility and the cytoskeleton, Volume 6, Issue 5, Pages 502-509.

The published version is available at http://dx.doi.org/10.1002/cm.970060509 .

Copyright © 1986 Wiley.

Citation Information
Annette T. Hastie, Susan T. Hingley, M. L. Higgins, Friedrich Kueppers, et al.. "Rhamnolipid from Pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes." Cell motility and the cytoskeleton Vol. 6 Iss. 5 (1986) p. 502 - 509
Available at: http://works.bepress.com/susan_hingley/33/