Rhamnolipid from Pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes.Cell motility and the cytoskeleton
AbstractIsolated ciliary axonemes from pig trachea were exposed to increasing concentrations of purified Pseudomonas aeruginosa rhamnolipid. This is a defined ciliary system allowing observation of direct impairment of functional axonemes. Axonemal motility and ATPase activity were decreased in proportion to rhamnolipid concentrations. ATPase-associated proteins observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dynein arms seen in ultra-structural cross sections progressively disappeared from axonemes with exposure to rhamnolipid. These four independent measures establish that the rhamnolipid removes the ATPase-containing outer dynein arms from the ciliary axoneme, thereby rendering the axoneme immotile.
Citation InformationAnnette T. Hastie, Susan T. Hingley, M. L. Higgins, Friedrich Kueppers, et al.. "Rhamnolipid from Pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes." Cell motility and the cytoskeleton Vol. 6 Iss. 5 (1986) p. 502 - 509
Available at: http://works.bepress.com/susan_hingley/33/