A foodborne illness outbreak can be costly to a retail foodservice operation. This research incorporated a mixed methods approach to develop an instrument for measuring retail foodservice employees’ motivational factors related to following food safety practices. Using exploratory design for instrument development, a sequential data collection and analysis approach was taken. Instrument pilot testing revealed a reliable instrument with 3 factors related to external motivation. Additional items were added to also assess internal motivators. The final tool contained 35 questions assessing internal and external motivational factors. Uses of the instrument to help identify employee motivators are discussed.