Skip to main content
Article
Parenchymal trafficking of pleural mesothelial cells in idiopathic pulmonary fibrosis.
Advanced Lung Disease and Lung Transplantation Articles
  • Kamal K. Mubarak
  • Ana Montes-Worboys
  • Doron Regev
  • N. Regev
  • Kamal A. Mohammed
  • I Faruqi
  • E. Fensel
  • Maher A. Baz
  • Olufemi A. Akindipe,
  • S. Fernandez-Bussy
  • Steven D. Nathan, Inova Fairfax Hospital
  • V. B. Anthony
Document Type
Article
Publication Date
1-1-2012
Abstract

Abstract

Idiopathic pulmonary fibrosis (IPF) is characterised by myofibroblast proliferation leading to architectural destruction. Neither the origin nor the continued proliferation of myofibroblasts is well understood. Explanted human IPF lungs were stained by immunohistochemistry for calretinin, a marker of pleural mesothelial cells (PMCs). Chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF) lungs acted as controls. The number of PMCs per 100 nucleated cells and per photomicrograph was estimated along with the Ashcroft score of fibrosis. Mouse PMCs expressing green fluorescent protein (GFP) or labelled with nanoparticles were injected into the pleural space of mice given intranasal transforming growth factor (TGF)-β1. Mouse lungs were lavaged and examined for the presence of GFP, smooth muscle α-actin (α-SMA) and calretinin. Calretinin-positive PMCs were found throughout IPF lungs, but not in COPD or CF lungs. The number of PMCs correlated with the Ashcroft score. In mice, nanoparticle-laden PMCs were recoverable by bronchoalveolar lavage, depending on the TGF-β1 dose. Fluorescent staining showed α-SMA expression in GFP-expressing PMCs, with co-localisation of GFP and α-SMA. PMCs can traffic through the lung and show myofibroblast phenotypic markers. PMCs are present in IPF lungs, and their number correlates with IPF severity. Since IPF presumably begins subpleurally, PMCs could play a pathogenetic role via mesothelial-mesenchymal transition

Citation Information
Kamal K. Mubarak, Ana Montes-Worboys, Doron Regev, N. Regev, et al.. "Parenchymal trafficking of pleural mesothelial cells in idiopathic pulmonary fibrosis." (2012)
Available at: http://works.bepress.com/steven_nathan/21/