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Oxalate metabolism by the acetogenic bacterium Moorella thermoacetica
FEMS Microbiology Letters (2004)
  • Steven L. Daniel, Eastern Illinois University
  • Christine Pilsl, University of Bayreuth
  • Harold L. Drake, University of Bayreuth

Whole-cell and cell-extract experiments were performed to study the mechanism of oxalate metabolism in the acetogenic bacterium Moorella thermoacetica. In short-term, whole-cell assays, oxalate consumption was low unless cell suspensions were supplemented with CO2, KNO3, or Na2S2O3. Cell extracts catalyzed the oxalate-dependent reduction of benzyl viologen. Oxalate consumption occurred concomitant to benzyl viologen reduction; when benzyl viologen was omitted, oxalate was not appreciably consumed. Based on benzyl viologen reduction, specific activities of extracts averaged 0.6 μmol oxalate oxidized min−1 mg protein−1. Extracts also catalyzed the formate-dependent reduction of NADP+; however, oxalate-dependent reduction of NADP+ was negligible. Oxalate- or formate-dependent reduction of NAD+ was not observed. Addition of coenzyme A (CoA), acetyl-CoA, or succinyl-CoA to the assay had a minimal effect on the oxalate-dependent reduction of benzyl viologen. These results suggest that oxalate metabolism by M. thermoacetica requires a utilizable electron acceptor and that CoA-level intermediates are not involved.

  • Moorella thermoacetica,
  • Acetyl-CoA pathway,
  • Oxalate,
  • Wood–Ljungdahl pathway,
  • Acetogenesis
Publication Date
February, 2004
Citation Information
Steven L. Daniel, Christine Pilsl and Harold L. Drake. "Oxalate metabolism by the acetogenic bacterium Moorella thermoacetica" FEMS Microbiology Letters Vol. 231 Iss. 1 (2004)
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