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Carbon Monoxide-Dependent Chemolithotrophic Growth of Clostridium thermoautotrophicum
  • M. Dean Savage
  • Zhongren Wu
  • Steven L. Daniel, Eastern Illinois University
  • Leon L. Lundie, JR
  • Harold L. Drake

The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

Publication Date
August, 1987
Citation Information
M. Dean Savage, Zhongren Wu, Steven L. Daniel, Leon L. Lundie, et al.. "Carbon Monoxide-Dependent Chemolithotrophic Growth of Clostridium thermoautotrophicum" APPLIED AND ENVIRONMENTAL MICROBIOLOGY Vol. 53 (1987)
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