Article
Enzyme Polymorphisms as Genetic Signatures in Human Cell Cultures
Science
Document Type
Article
Publication Date
3-25-1977
Disciplines
Abstract
The electrophoretic resolution of seven relatively polymorphic human gene-enzyme systems expressed in tissue culture cells can be used as a sensitive genetic monitor for intraspecific cell contamination. An identical genotype at each of the same allozyme loci provides a 95 percent (or greater) confidence estimate of the identity of two cultured lines, on the basis of the allelic frequencies of the seven enzyme loci in natural populations and in populations of independently derived cultured cells. Of 27 commonly used human cell lines examined, only one of 351 pairwise comparisons proved genetically indistinguishable.
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
DOI
10.1126/science.841332
Citation Information
Stephen J. O'Brien, Gail Kleiner, Russell Olson and John E. Shannon. "Enzyme Polymorphisms as Genetic Signatures in Human Cell Cultures" Science Vol. 195 Iss. 4284 (1977) p. 1345 - 1348 ISSN: 0036-8075 Available at: http://works.bepress.com/stephen-obrien/216/
©1977 American Association for the Advancement of Science