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Cloning and Chromosome Mapping of the Feline Genes p21WAF1 and p27Kip1
Gene
  • Masaru Okuda, University of Tokyo
  • Ken-ichi Minehata, University of Tokyo
  • Asuka Setoguchi, University of Tokyo
  • Kyu-Woan Cho, University of Tokyo
  • Noriko Nakamura, University of Tokyo
  • Kazuo Nishigaki, University of Tokyo
  • Toshihiro Watari, University of Tokyo
  • Stanley Cevario, National Cancer Institute at Frederick
  • Stephen J. O'Brien, National Cancer Institute at Frederick
  • Hajime Tsujimoto, University of Tokyo
  • Atsuhiko Hasegawa, University of Tokyo
Document Type
Article
Publication Date
10-1-1997
Keywords
  • Cyclin-dependent kinase inhibitors,
  • Feline tumor,
  • Molecular cloning,
  • Chromosomal mapping
Abstract

For investigation of the relation of cell cycle regulation with tumorigenesis in cats, we carried out molecular cloning of feline p21WAF1 and p27Kip1 cDNAs and chromosomal mapping of these genes on the cat genome. The feline p21WAF1 cDNA clone obtained in this study encoded 164 amino acids (aa) showing 83.5% and 76.8% sequence similarity with those of the human and mouse counterparts, respectively. The cat p27Kip1 cDNA clone isolated here encoded 198 aa, showing sequence similarities of 93.4% and 90.4% with its human and mouse counterparts, respectively. Using a panel of feline×rodent somatic cell hybrids, the feline CDKN1A (p21WAF1) and CDKN1B(p27Kip1) loci were assigned to feline chromosomes B2 and B4, respectively. Southern-blot analyses of 17 feline spontaneous leukemia and lymphoma cases using these cDNAs as probes did not reveal any rearrangements in either the p21WAF1 or the p27Kip1 gene. RT-PCR/SSCP (single strand conformation polymorphism) analysis of p27Kip1 cDNA did not uncover any amino acid substitutions in the 10 feline leukemia and lymphoma cases that were examined.

Comments

© 1997 Elsevier Science B.V.

Additional Comments
GenBank accession #s: D84649-D84650
ORCID ID
0000-0001-7353-8301
ResearcherID
N-1726-2015
Citation Information
Masaru Okuda, Ken-ichi Minehata, Asuka Setoguchi, Kyu-Woan Cho, et al.. "Cloning and Chromosome Mapping of the Feline Genes p21WAF1 and p27Kip1" Gene Vol. 198 Iss. 1-2 (1997) p. 141 - 147 ISSN: 0378-1119
Available at: http://works.bepress.com/stephen-obrien/126/