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Mucosa-Associated Bacterial Microbiome of the Gastrointestinal Tract of Weaned Pigs and Dynamics Linked to Dietary Calcium-Phosphorus
PLOS ONE (2014)
  • Evelyne Mann, Vetmeduni Vienna
  • Stephan Schmitz-Esser, Vetmeduni Vienna
  • Qendrim Zebeli, Vetmeduni Vienna
  • Martin Wagner, Vetmeduni Vienna
  • Mathias Ritzmann, Vetmeduni Vienna
  • Barbara U. Metzler-Zebeli, Vetmeduni Vienna
Abstract
Dietary composition largely influences pig’s gastrointestinal microbiota and represents a useful prophylactic tool against enteric disturbances in young pigs. Despite the importance for host-microbe interactions and bacterial colonization, dietary responses of the mucosa-associated bacterial communities are less well investigated. In the present study, we characterized the mucosa-associated bacterial communities at the Pars non-glandularis of the stomach, ileum and colon, and identified shifts in these communities in response to different dietary calcium-phosphorus (Ca-P) contents (100% versus 190% of the Ca and P requirements) in combination with two basal diets (wheat-barley- or corn-based) in weaned pigs. Pyrosequencing of 16S rRNA genes from 93 mucosal samples yielded 447,849 sequences, clustering into 997 operational taxonomic units (OTUs) at 97% similarity level. OTUs were assigned to 198 genera belonging to 14 different phyla. Correlation-based
networks revealed strong interactions among OTUs at the various gastrointestinal sites. Our data describe a previously not reported high diversity and species richness at the Pars non-glandularis of the stomach in weaned pigs. Moreover, high versus adequate Ca-P content significantly promoted Lactobacillus by 14.9% units (1.4 fold change) at the gastric Pars nonglandularis (P = 0.035). Discriminant analysis revealed dynamic changes in OTU composition in response to dietary cereals and Ca-P contents at all gastrointestinal sites which were less distinguishable at higher taxonomic levels. Overall, this study revealed a distinct mucosa-associated bacterial community at the different gut sites, and a strong effect of high Ca-P diets on the gastric community, thereby markedly expanding our comprehension on mucosa-associated microbiota and their diet-related dynamics in weaned pigs.
Publication Date
January, 2014
DOI
10.1371/journal.pone.0086950
Publisher Statement
Copyright:  2014 Mann et al.
Citation Information
Evelyne Mann, Stephan Schmitz-Esser, Qendrim Zebeli, Martin Wagner, et al.. "Mucosa-Associated Bacterial Microbiome of the Gastrointestinal Tract of Weaned Pigs and Dynamics Linked to Dietary Calcium-Phosphorus" PLOS ONE Vol. 9 Iss. 1 (2014) p. e86950
Available at: http://works.bepress.com/stephan-schmitz-esser/11/
Creative Commons license
Creative Commons License
This work is licensed under a Creative Commons CC_BY International License.