Expression of an antigen receptor on T cells does not require recombination at the immunoglobulin JH-C mu locusWomen’s Health Research Faculty Publications
UMMS AffiliationDepartment of Molecular Genetics and Microbiology
SubjectsAnimals; Cell Line; Gene Expression Regulation; Genes; Immunoglobulin Heavy Chains; Immunoglobulin mu-Chains; Mice; Mice, Inbred Strains; Nucleic Acid Hybridization; Receptors, Antigen, T-Cell; Recombination, Genetic; T-Lymphocytes
AbstractConsiderable evidence has accumulated suggesting that the antigen receptor(s) on T cells is coded for by genes for the variable (V) region of the immunoglobulin heavy (H) chains. In B cells, a complete gene for the immunoglobulin VH region is formed by somatic recombination of VH and joining region heavy chain (JH) gene segments [through an intermediate diversity(D) region gene segment]. In an attempt to determine whether a complete immunoglobulin VH region is expressed on T cells that bear an antigen receptor, we analyzed the restriction map of the JH-C mu locus in genomic DNA from two cloned murine cytotoxic T-lymphocyte (CTL) lines specific for the x-ray-induced leukemia RL male 1. We found no rearrangement of the JH C mu locus in the CTL lines, indicating that the T-cell antigen receptor(s) in these CTLs is not coded for by a complete immunoglobulin VH gene formed by joining of VH, (DH), and JH genes. In addition, we determined that C mu genes on both chromosomes were present and that there was no rearrangement of the C alpha, C kappa, or lambda chain genes in these CTL cells.
SourceProc Natl Acad Sci U S A. 1981 Jun;78(6):3814-8.
Related ResourcesLink to article in PubMed
Citation InformationY. Cayre, M. A. Palladino, K. B. Marcu and Janet Stavnezer. "Expression of an antigen receptor on T cells does not require recombination at the immunoglobulin JH-C mu locus" Vol. 78 Iss. 6 (1981) ISSN: 0027-8424 (Print)
Available at: http://works.bepress.com/stavnezerj/29/