Article
36 Degrees Step Size of Proton-driven C-ring Rotation in FoF1-ATP Synthase
EMBO Journal
Document Type
Article
Publication Date
9-16-2009
URL with Digital Object Identifier
http://dx.doi.org/10.1038/emboj.2009.213
Disciplines
Abstract
Synthesis of adenosine triphosphate ATP, the 'biological energy currency', is accomplished by F(o)F(1)-ATP synthase. In the plasma membrane of Escherichia coli, proton-driven rotation of a ring of 10 c subunits in the F(o) motor powers catalysis in the F(1) motor. Although F(1) uses 120 degrees stepping during ATP synthesis, models of F(o) predict either an incremental rotation of c subunits in 36 degrees steps or larger step sizes comprising several fast substeps. Using single-molecule fluorescence resonance energy transfer, we provide the first experimental determination of a 36 degrees sequential stepping mode of the c-ring during ATP synthesis.
Citation Information
Monika G. Düser, Nawid Zarrabi, Daniel J. Cipriano, Stefan Ernst, et al.. "36 Degrees Step Size of Proton-driven C-ring Rotation in FoF1-ATP Synthase" EMBO Journal Vol. 28 Iss. 18 (2009) p. 2689 - 2696 Available at: http://works.bepress.com/stanley_dunn/1/